بررسی اثر عصاره‌ی هیدروالکلی گیاه پسیدیوم گواوا بر سلول‌های خونی در موش صحرایی نر
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نگین عالی
سیما سراوانی
حلیمه عالی مهرانگیز غفاری مریم مجاهدتقی الهه حقیقت منش
سیما سراوانی
حلیمه عالی مهرانگیز غفاری مریم مجاهدتقی الهه حقیقت منش
چکیده
مقدمه: استفاده از گياهان دارویی و تركيبات آن‌ها از زمان‌هاي گذشته براي درمان بيماری‌ها مورد استفاده قرار گرفته است. از جمله به دلیل وجود آلکالوئیدها و ترکیبات فنلی در عصاره‌ی گیاه گواوا و با وجود تحقيقاتی که در مورد خواص این گیاه انجام شده و گسترش بیماری‌های خونی، ولي در مورد اثرات آن، بر روی سلول‌های خونی، تحقيقات کمتری صورت گرفته است. لذا ما را بر آن داشت تا، اثر عصاره‌ی هیدروالکلی برگ گیاه پسیدیوم گواوا را بر روی سلول‌های خونی در موش صحرایی بررسی کنیم.
شیوه‌ی مطالعه: پس از عصاره‌گیری برگ گیاه گواوا، ۴۰ سر موش صحرایی بالغ نر به چهار گروه تقسیم شدند. که شامل شاهد، (حیواناتی که، در طول مطالعه، درمانی در آن‌ها صورت نگرفت) و گروه‌های آزمایش (حیواناتی که، به مدت 3 ماه، عصاره‌ی هیدروالکلی برگ پسیدیوم گواوا با دوزهای مختلف، به صورت روزانه دریافت کردند) قرار گرفتند. پس از پایان دوره‌ی آزمایش، شمارش گلبول‌های قرمز، سفید، هموگلوبین، هماتوکریت و پلاکت‌های خون انجام گرفت.
یافته‌ها: نتایج حاصل از این مطالعه نشان داد که عصاره‌ی هیدروالکلی افزایش دهنده‌ی گلبول‌های سفید خون و گلبول قرمز بوده است اما تغییر معنی‌دار مشاهده نشد، که این افزایش در میزان هماتوکریت در گروه دریافت کننده‌ی عصاره‌ی هیدروالکلی با دوز 5000 میلی‌گرم بر کیلوگرم نسبت به گروه شاهد معنی‌دار می‌باشد.
نتیجه‌گیری: نتیجه‌ی این مطالعه، تأثیر مثبتی بر روی سلول‌های خونی نشان داد. همچنین عدم سمیت سلولی با دوزهای بالای عصاره‌ی این گیاه به معنای استفاده‌ی ایمن از آن در طب سنتی است.
واژگان کلیدی: پسیدیوم گواوا، سلول‌های خون، موش صحرایی
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استناد به مقاله
عالین., سراوانیس., عالیح., غفاریم., مجاهدتقیم., & حقیقت منشا. (2020). بررسی اثر عصاره‌ی هیدروالکلی گیاه پسیدیوم گواوا بر سلول‌های خونی در موش صحرایی نر. مجله دانشکده پزشکی زابل, 167-175. Retrieved از http://jzms.ir/index.php/JZMS/article/view/234
نوع مقاله
مقاله پژوهشی
مراجع
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11. de Oliveira Teixeira R, Camparoto ML, Mantovani MS, Pimenta Vicentini VE. Assessment of two medicinal plants, Psidium guajava L. and Achillea millefolium L., in in vitro and in vivo assays. Genet Mol Biol 2003; 26(4): 551-5.
12. Holetz FB, Pessini GL, Sanches NR, Cortez DAG, Nakamura CV, Dias Filho BP. Screening of some plants used in the Brazilian folk medicine for the treatment of infectious diseases. Mem Inst Oswaldo Cruz 2002; 97(7): 1027-31.
13. Abreu PRC, Almeida MC, Bernardo RM, Brito LC, Garcia EAC, Fonseca AS, et al. Guava extract (Psidium guajava) alters the labelling of blood constituents with technetium-99m. J Zhejiang Univ Sci B 2006; 7(6): 429-35.
14. Kawakami Y, Hosokawa T, Suzuki-Yamamoto T, Yamashita H, Kimoto M, Tsuji H. Antiproliferative activity of guava leaf extract via inhibition of, prostaglandin endoperoxide H synthase isoforms. Prostaglandins Leukot Essent Fatty Acids 2009; 80(5-6): 239-45.
15. Murray RK. Harpers’s biochemistry. In: Harper's illustrated biochemistry. Rodwell VW, Bender DA, Botham KM, Kennelly PJ, Weil PA. editors. New York, NY: McGraw-Hill; 2000. p. 780-6.
16. Zaeemi Baravati Sh, Shahanipoor K, Monajemi R. Evaluation of cytotoxic effect of aqueous and hydro alcoholic extracts of guava fruit psidium guajava l. On cell line du-145. Journal of Animal Physiology and Development 2015; 8(3): 69-77. [In Persian].
17. Saravani S, Tanideh N, Fazeli M, Azarpira N, Nadimi E, Lotfi M, et al. Evaluation of hydroalcoholic extract of Psidium Guajava Linn.(Myrtaceae) leave effect with synovium-derived Stem Cells and Platelet Rich Plasma (PRP) on induced osteoarthritis of the knee in Male Rat. Iranian Journal of Orthopaedic Surgery 2017; 15(2): 28-38.
18. Sanches NR, Garcia Cortez DA, Schiavini MS, Nakamura CV, Dias Filho BP. An evaluation of antibacterial activities of Psidium guajava (L.). Braz Arch Biol Technol 2005; 48(3): 429-36.
19. Sulain MD, Zazali KE, Ahmad N. Screening on anti-proliferative activity of Psidium guajava leaves extract towards selected cancer cell lines. J US-China Med Sci 2012; 9(1): 30-7.
20. Jaiarj P, Khoohaswan P, Wongkrajang Y, Peungvicha P, Suriyawong P, Saraya ML, et al. Anticough and antimicrobial activities of Psidium guajava Linn. leaf extract. J Ethnopharmacol 1999; 67(2): 203-12.
21. Manosroi J, Dhumtanom P, Manosroi A. Anti-proliferative activity of essential oil extracted from Thai medicinal plants on KB and P388 cell lines. Cancer Letters 2006; 235(1):114-20.
22. Extracta liquida pharmaceutical formulations. In: Le G, editor. The chemist and druggist book. 11th ed. London: Essex Street Strand; 1950. p. 183.
23. Akbari A, Nasiri K, Heydari M, Mosavat SH, Iraji A. The protective effect of hydroalcoholic extract of zingiber officinale roscoe (ginger) on ethanol-induced reproductive toxicity in male rats. J Evid Based Complementary Altern Med 2017; 22(4): 609-617.
24. Dabbaghmanesh MH, Noorafshan A, Talezadeh P, Tanideh N, Koohpeyma F, Iraji A, et al. Stereological investigation of the effect of Elaeagnus angustifolia fruit hydroalcoholic extract on osteoporosis in ovariectomized rats. Avicenna J Phytomed 2017; 7(3): 261-74.
25. Kaileh M, Berghe WV, Boone E, Essawi T, Haegeman G. Screening of indigenous Palestinian medicinal plants for potential anti-inflammatory and cytotoxic activity. J Ethnopharmacol 2007; 113(3): 510-6.
26. Limsong J, Benjavongkulchai E, Kuvatanasuchati J. Inhibitory effect of some herbal extracts on adherence of Streptococcus mutans. J
Eethnopharmacol 2004; 92(2-3): 281-9.
27. Chen KC, Hsieh CL, Peng CC, Hsieh-Li HM, Chiang HS, Huang KD, et al. Brain derived metastatic prostate cancer DU-145 cells are effectively inhibited in vitro by guava (Psidium gujava L.) leaf extracts. Nutr Cancer 2007; 58(1): 93-106.
28. Mirazi N, Gholami M. Study of protective effect of Avicennia marina leaf hydroalchoholic extract on bone marrow tissue in male rate induced with CCl4. J Arak Univ Med Sci 2016; 19(8): 88-98. [In Persian].
29. Modaresi M, Resalatpour N. The effect of taraxacum officinale hydro alcoholic extract on the blood cell counts in mice. Armaghan Danesh 2013; 17(5): 431-8. [In Persian].
30. Abdanipour A, Nejatbakhsh R, Jafari Anarkooli I, Ghorbanlo M, Nikfar A, Noriyan A. Prolifration and prolifration and anti-apoptoWLF effect of hydroethanolic extract of lavandula officinalis on rat neural stem cells. J Adv Med Biomed Res 2016; 24(105): 43-52. [In Persian].
31. Uboh FE, Okon IE, Ekong MB. Effect of Aqueous Extract of Psidium Guajava Leaves on Liver Enzymes, Histological Integrity and
Hematological Indices in Rats. Gastroenterology Res 2010; 3(1): 32-38.
32. Adeyemi O, Akanji MA, Ekanem JT. Anti-anaemic properties of the ethanolic extracts ofPsidium guajava, in Trypanosoma brucei bruceiInfected Rats. Res J Pharmacol 2010; 4(3): 74-7.
33. Udem SC, Anyanwu MU, Obidike RI, Udem ND. The effects of Psidium guajava Linn. (Myrtaceae) leaf, chloroform extract on some hematological and biochemical parameters in mice. Comp Clin Pathol 2011; 20: 47-51.
34. Madduluri S, Sitaram B. In vitro evaluation of anti inflammatory activity of methanolic and ethanolic leaf, extracts of five indigenous plants in South India. Int J Pharm Tech Res 2014; 6(2): 569-74.
35. Modaresi M, Asadi S. The effect of Echinacea Purpura hydroalcoholic extract on the blood parameters in mice. Yafte 2012; 14(2): 43-9.
[In Persian].
36. Modaresi M. Effect of Angelica archangelica root hydro-alcoholic extract on the blood cells of small laboratory mice. Horizon Med Sci 2013; 18(4): 149-53.
2. Ochoa FL, Márquez Alonso C. Plantas Medicinales de México: Composición, Usos y Actividad Biológica [Medicinal Plants of Mexico: Composition, Uses and Biological Activity]. Mexico City, Mexico: Universidad Nacional Autónoma de México; 1996. p. 135.
3. Heinrich M, Ankli A, Frei B, Weimann C, Sticher O. Medicinal plants in Mexico: Healers' consensus and cultural importance. Soc Sci Med 1998; 47(11): 1859-71.
4. Aguilar A, Argueta A, Cano L. Flora Medicinal indigena de mexico. treinta y cinco monografias del atlas de las plantas de la medicina tradicional Mexicana. Campeche, San Cristóbal: Biblioteca De La Medicina Tradicional Mexicana; 1994.
5. Oh WK, Lee CH, Lee MS, Bae EY, Sohn CB, Oh H, et al. Antidiabetic effects of extracts from Psidium guajava. Journal of Ethnopharmacology 2005; 96(3): 411-5.
6. Ojewole JAO. Hypoglycemic and hypotensive effects of Psidium guajava Linn.(Myrtaceae) leaf aqueous extract. Methods Find Exp Clin Pharmacol 2005; 27(10): 689-95.
7. Conway P. Tree medicine: a comprehensive guide to the healing power of over 170 trees. Boston, US: Piatkus Books; 2002.
8. Cabieses F. Apuntes de medicina tradicional: La racionalización de lo irracional. Apuntes de medicina tradicional: La racionalización de lo irracional: Talleres A&B; 1993.
9. Smith NJ, Williams JT, Plucknett DL, Talbot JP. Tropical forests and their crops. Ithaca, NY: Cornell University Press; 1992.
10. Morton JF. Fruits of warm climates. Winterville, N.C: Creative Resource Systems, Inc.; 1987.p. 356-63.
11. de Oliveira Teixeira R, Camparoto ML, Mantovani MS, Pimenta Vicentini VE. Assessment of two medicinal plants, Psidium guajava L. and Achillea millefolium L., in in vitro and in vivo assays. Genet Mol Biol 2003; 26(4): 551-5.
12. Holetz FB, Pessini GL, Sanches NR, Cortez DAG, Nakamura CV, Dias Filho BP. Screening of some plants used in the Brazilian folk medicine for the treatment of infectious diseases. Mem Inst Oswaldo Cruz 2002; 97(7): 1027-31.
13. Abreu PRC, Almeida MC, Bernardo RM, Brito LC, Garcia EAC, Fonseca AS, et al. Guava extract (Psidium guajava) alters the labelling of blood constituents with technetium-99m. J Zhejiang Univ Sci B 2006; 7(6): 429-35.
14. Kawakami Y, Hosokawa T, Suzuki-Yamamoto T, Yamashita H, Kimoto M, Tsuji H. Antiproliferative activity of guava leaf extract via inhibition of, prostaglandin endoperoxide H synthase isoforms. Prostaglandins Leukot Essent Fatty Acids 2009; 80(5-6): 239-45.
15. Murray RK. Harpers’s biochemistry. In: Harper's illustrated biochemistry. Rodwell VW, Bender DA, Botham KM, Kennelly PJ, Weil PA. editors. New York, NY: McGraw-Hill; 2000. p. 780-6.
16. Zaeemi Baravati Sh, Shahanipoor K, Monajemi R. Evaluation of cytotoxic effect of aqueous and hydro alcoholic extracts of guava fruit psidium guajava l. On cell line du-145. Journal of Animal Physiology and Development 2015; 8(3): 69-77. [In Persian].
17. Saravani S, Tanideh N, Fazeli M, Azarpira N, Nadimi E, Lotfi M, et al. Evaluation of hydroalcoholic extract of Psidium Guajava Linn.(Myrtaceae) leave effect with synovium-derived Stem Cells and Platelet Rich Plasma (PRP) on induced osteoarthritis of the knee in Male Rat. Iranian Journal of Orthopaedic Surgery 2017; 15(2): 28-38.
18. Sanches NR, Garcia Cortez DA, Schiavini MS, Nakamura CV, Dias Filho BP. An evaluation of antibacterial activities of Psidium guajava (L.). Braz Arch Biol Technol 2005; 48(3): 429-36.
19. Sulain MD, Zazali KE, Ahmad N. Screening on anti-proliferative activity of Psidium guajava leaves extract towards selected cancer cell lines. J US-China Med Sci 2012; 9(1): 30-7.
20. Jaiarj P, Khoohaswan P, Wongkrajang Y, Peungvicha P, Suriyawong P, Saraya ML, et al. Anticough and antimicrobial activities of Psidium guajava Linn. leaf extract. J Ethnopharmacol 1999; 67(2): 203-12.
21. Manosroi J, Dhumtanom P, Manosroi A. Anti-proliferative activity of essential oil extracted from Thai medicinal plants on KB and P388 cell lines. Cancer Letters 2006; 235(1):114-20.
22. Extracta liquida pharmaceutical formulations. In: Le G, editor. The chemist and druggist book. 11th ed. London: Essex Street Strand; 1950. p. 183.
23. Akbari A, Nasiri K, Heydari M, Mosavat SH, Iraji A. The protective effect of hydroalcoholic extract of zingiber officinale roscoe (ginger) on ethanol-induced reproductive toxicity in male rats. J Evid Based Complementary Altern Med 2017; 22(4): 609-617.
24. Dabbaghmanesh MH, Noorafshan A, Talezadeh P, Tanideh N, Koohpeyma F, Iraji A, et al. Stereological investigation of the effect of Elaeagnus angustifolia fruit hydroalcoholic extract on osteoporosis in ovariectomized rats. Avicenna J Phytomed 2017; 7(3): 261-74.
25. Kaileh M, Berghe WV, Boone E, Essawi T, Haegeman G. Screening of indigenous Palestinian medicinal plants for potential anti-inflammatory and cytotoxic activity. J Ethnopharmacol 2007; 113(3): 510-6.
26. Limsong J, Benjavongkulchai E, Kuvatanasuchati J. Inhibitory effect of some herbal extracts on adherence of Streptococcus mutans. J
Eethnopharmacol 2004; 92(2-3): 281-9.
27. Chen KC, Hsieh CL, Peng CC, Hsieh-Li HM, Chiang HS, Huang KD, et al. Brain derived metastatic prostate cancer DU-145 cells are effectively inhibited in vitro by guava (Psidium gujava L.) leaf extracts. Nutr Cancer 2007; 58(1): 93-106.
28. Mirazi N, Gholami M. Study of protective effect of Avicennia marina leaf hydroalchoholic extract on bone marrow tissue in male rate induced with CCl4. J Arak Univ Med Sci 2016; 19(8): 88-98. [In Persian].
29. Modaresi M, Resalatpour N. The effect of taraxacum officinale hydro alcoholic extract on the blood cell counts in mice. Armaghan Danesh 2013; 17(5): 431-8. [In Persian].
30. Abdanipour A, Nejatbakhsh R, Jafari Anarkooli I, Ghorbanlo M, Nikfar A, Noriyan A. Prolifration and prolifration and anti-apoptoWLF effect of hydroethanolic extract of lavandula officinalis on rat neural stem cells. J Adv Med Biomed Res 2016; 24(105): 43-52. [In Persian].
31. Uboh FE, Okon IE, Ekong MB. Effect of Aqueous Extract of Psidium Guajava Leaves on Liver Enzymes, Histological Integrity and
Hematological Indices in Rats. Gastroenterology Res 2010; 3(1): 32-38.
32. Adeyemi O, Akanji MA, Ekanem JT. Anti-anaemic properties of the ethanolic extracts ofPsidium guajava, in Trypanosoma brucei bruceiInfected Rats. Res J Pharmacol 2010; 4(3): 74-7.
33. Udem SC, Anyanwu MU, Obidike RI, Udem ND. The effects of Psidium guajava Linn. (Myrtaceae) leaf, chloroform extract on some hematological and biochemical parameters in mice. Comp Clin Pathol 2011; 20: 47-51.
34. Madduluri S, Sitaram B. In vitro evaluation of anti inflammatory activity of methanolic and ethanolic leaf, extracts of five indigenous plants in South India. Int J Pharm Tech Res 2014; 6(2): 569-74.
35. Modaresi M, Asadi S. The effect of Echinacea Purpura hydroalcoholic extract on the blood parameters in mice. Yafte 2012; 14(2): 43-9.
[In Persian].
36. Modaresi M. Effect of Angelica archangelica root hydro-alcoholic extract on the blood cells of small laboratory mice. Horizon Med Sci 2013; 18(4): 149-53.